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ho1  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc ho1
    Ho1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ho1/pm41794516-35-5-10
    Average 86 stars, based on 1 article reviews
    ho1 - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Fibroblast growth factor 19 regulates polycystic ovary syndrome progression via FGFR4-ERK-NRF2 pathway
    Article Snippet: .. After blocking with 5% skim milk at 37 °C for 1 h, the membranes were incubated with primary antibodies against NRF2 (cat. no. ab89443, 1: 1000, Abcam), FGFR4 (cat. no. 11098-1-AP; 1:1000; Proteintech) and FLAG (cat. no. 80010-1-RR, 1:10000, Proteintech); ERK (cat. no. 11257-1-AP; 1:1000; Proteintech) and BAX (cat. no. 50599-2-Ig; 1:1000; Proteintech) and p-ERK (cat. no. #4695, 1:1000; Cell Signaling Technology) and BCL2 (cat. no. A0208, 1:1000; Abcam), HO1 (cat. no. 82206, 1:1000, Cell Signaling Technology) and GAPDH (cat. no. #5174, 1:20000, Cell Signaling Technology) overnight at 4 °C. .. After washing, the membranes were incubated with goat anti-mouse/rabbit IgG (H + L)-HRP (cat. no. #7076 or #7074, 1:5000, Cell Signaling Technology) at 37 °C for 2 h. Finally, the immunoreactive bands were detected using a Millipore enhanced chemiluminescence (ECL) detection kit (Beyotime).

    Incubation:

    Article Title: Fibroblast growth factor 19 regulates polycystic ovary syndrome progression via FGFR4-ERK-NRF2 pathway
    Article Snippet: .. After blocking with 5% skim milk at 37 °C for 1 h, the membranes were incubated with primary antibodies against NRF2 (cat. no. ab89443, 1: 1000, Abcam), FGFR4 (cat. no. 11098-1-AP; 1:1000; Proteintech) and FLAG (cat. no. 80010-1-RR, 1:10000, Proteintech); ERK (cat. no. 11257-1-AP; 1:1000; Proteintech) and BAX (cat. no. 50599-2-Ig; 1:1000; Proteintech) and p-ERK (cat. no. #4695, 1:1000; Cell Signaling Technology) and BCL2 (cat. no. A0208, 1:1000; Abcam), HO1 (cat. no. 82206, 1:1000, Cell Signaling Technology) and GAPDH (cat. no. #5174, 1:20000, Cell Signaling Technology) overnight at 4 °C. .. After washing, the membranes were incubated with goat anti-mouse/rabbit IgG (H + L)-HRP (cat. no. #7076 or #7074, 1:5000, Cell Signaling Technology) at 37 °C for 2 h. Finally, the immunoreactive bands were detected using a Millipore enhanced chemiluminescence (ECL) detection kit (Beyotime).

    Article Title: Methylene Blue Attenuates 3-Nitropropionic Acid-Induced Oxidative Stress and Mitochondrial Dysfunction in Striatal Cells: Therapeutic Implications in Huntington's Disease Neuropathology.
    Article Snippet: .. Non-specific binding sites were blocked with 3% BSA for 1 h at room temperature, and the membranes were incubated overnight at 4 ◦C with primary antibodies directed against HO1, SOD1, SOD2, KEAP1, NRF2, p62, Fis1, DRP1, OPA1, PGC1-α, and β-Actin (Cell Signaling Technology, Danvers, MA, USA). .. Blots were incubated with appropriate HRP-conjugated secondary antibodies, and bands were visualized using luminol-based chemiluminescent substrates (Thermo Scientific, Waltham, MA, USA) and imaged using a ChemiDoc Touch imaging system (BioRad, Hercules, CA, USA).

    Article Title: Methylene Blue Attenuates 3-Nitropropionic Acid-Induced Oxidative Stress and Mitochondrial Dysfunction in Striatal Cells: Therapeutic Implications in Huntington’s Disease Neuropathology
    Article Snippet: .. Non-specific binding sites were blocked with 3% BSA for 1 h at room temperature, and the membranes were incubated overnight at 4 °C with primary antibodies directed against HO1, SOD1, SOD2, KEAP1, NRF2, p62, Fis1, DRP1, OPA1, PGC1-ɑ, and β-Actin (Cell Signaling Technology, Danvers, MA, USA). .. Blots were incubated with appropriate HRP-conjugated secondary antibodies, and bands were visualized using luminol-based chemiluminescent substrates (Thermo Scientific, Waltham, MA, USA) and imaged using a ChemiDoc Touch imaging system (BioRad, Hercules, CA, USA).

    Binding Assay:

    Article Title: Methylene Blue Attenuates 3-Nitropropionic Acid-Induced Oxidative Stress and Mitochondrial Dysfunction in Striatal Cells: Therapeutic Implications in Huntington's Disease Neuropathology.
    Article Snippet: .. Non-specific binding sites were blocked with 3% BSA for 1 h at room temperature, and the membranes were incubated overnight at 4 ◦C with primary antibodies directed against HO1, SOD1, SOD2, KEAP1, NRF2, p62, Fis1, DRP1, OPA1, PGC1-α, and β-Actin (Cell Signaling Technology, Danvers, MA, USA). .. Blots were incubated with appropriate HRP-conjugated secondary antibodies, and bands were visualized using luminol-based chemiluminescent substrates (Thermo Scientific, Waltham, MA, USA) and imaged using a ChemiDoc Touch imaging system (BioRad, Hercules, CA, USA).

    Article Title: Methylene Blue Attenuates 3-Nitropropionic Acid-Induced Oxidative Stress and Mitochondrial Dysfunction in Striatal Cells: Therapeutic Implications in Huntington’s Disease Neuropathology
    Article Snippet: .. Non-specific binding sites were blocked with 3% BSA for 1 h at room temperature, and the membranes were incubated overnight at 4 °C with primary antibodies directed against HO1, SOD1, SOD2, KEAP1, NRF2, p62, Fis1, DRP1, OPA1, PGC1-ɑ, and β-Actin (Cell Signaling Technology, Danvers, MA, USA). .. Blots were incubated with appropriate HRP-conjugated secondary antibodies, and bands were visualized using luminol-based chemiluminescent substrates (Thermo Scientific, Waltham, MA, USA) and imaged using a ChemiDoc Touch imaging system (BioRad, Hercules, CA, USA).



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